亚博棋牌

Test Id :WNS

订购此测试

West Nile Virus Antibody, IgG and IgM, Serum

Download
Overview

亚博棋牌

Useful For
建议在测试可能有帮助的临床障碍或设置

Laboratory diagnosis of infection with West Nile virus in serum specimens

Highlights

Detection of antibodies to West Nile virus (WNV) in serum can be used to support the diagnosis of recent WNV infection.

该测试应仅用于诊断目的。

档案信息
A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test Id Reporting Name Available Separately 总是执行
WNGS West Nile Virus Ab, IgG, S 不 Yes
WNMS West Nile Virus Ab, IgM, S 不 Yes
WNVSI 西尼罗河血清解释 不 Yes

Testing Algorithm
描述将测试添加到初始顺序时的情况。这包括反射和其他测试。

以下算法可在特殊说明中获得：

-脑膜炎/脑膜炎算法

-Mosquito-borne Disease Laboratory Testing

Special Instructions
PDF库，包括与测试相关的相关信息和表格

Method Name
对执行测试的方法的简短描述

Enzyme-Linked Immunosorbent Assay (ELISA)

NY State Available
指示纽约州批准的状态以及纽约州客户的测试是否可订购。

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

西尼罗河病毒AB，IgG和IgM，S

Aliases
列出测试的其他通用名称，以帮助搜索

Arbovirus

Flavivirus

蚊子出现脑炎

病毒脑炎

西尼罗河病毒（WNV）

Testing Algorithm
描述将测试添加到初始顺序时的情况。这包括反射和其他测试。

以下算法可在特殊说明中获得：

-脑膜炎/脑膜炎算法

-Mosquito-borne Disease Laboratory Testing

标本类型
Describes the specimen type validated for testing

Serum

需要标本
定义执行测试所需的最佳标本和首选卷以完成测试

收集容器/管：

Preferred:Serum gel

可接受：Red top

标本卷：0.5 mL

Special Instructions
PDF库，包括与测试相关的相关信息和表格

Forms

If not ordering electronically, complete, print, and send aMicrobiology Test Request（T244）带试样。

标本Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.4毫升

拒绝
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
脂肪血症 Reject
大黄色 Reject
热灭活标本 Reject

标本Stability Information
提供了将样品运输到表演实验室所需的温度的描述，还包括可接受的温度

标本类型 温度 Time Special Container
Serum 冷藏（首选） 14 days
冷冻 14 days

Useful For
建议在测试可能有帮助的临床障碍或设置

Laboratory diagnosis of infection with West Nile virus in serum specimens

Testing Algorithm
描述将测试添加到初始顺序时的情况。这包括反射和其他测试。

以下算法可在特殊说明中获得：

-脑膜炎/脑膜炎算法

-Mosquito-borne Disease Laboratory Testing

临床信息
讨论与实验室测试有关的生理学，病理生理学和一般临床方面

西尼罗河病毒（WNV）是一种蚊子传播的黄病毒（单链RNA），主要感染鸟类，但也可以感染人类和马匹。WNV于1937年首次与乌干达西尼罗河地区的受感染者隔离。直到1999年在纽约市的鸟类中识别出病毒感染之前，WNV才在东半球发现，在非洲，亚洲，中东和欧洲的分布广泛。（1-3）在2012年，总共有。5674例WNV病例已报告给疾病控制与预防中心（CDC），其中2873（51％）被归类为神经侵染疾病（例如，脑膜炎或脑膜炎），286例（5％）（5％）导致死亡。（2）

Most people who are infected with WNV will not develop clinical signs of illness. It is estimated that about 20% of those who become infected will develop West Nile fever with mild symptoms, including fever, headache, myalgia, and occasionally a skin rash on the trunk of the body. Case fatality rates among patients hospitalized during recent outbreaks have ranged from 4% to 14%. Advanced age is the most important risk factor for death, and patients older than 70 years of age are at particularly high risk.(1)

通过证明特定的IgG和血清标本中的IgM类抗体，可以最好地实现实验室诊断。聚合酶链反应（PCR）测试（WNVP / West Nile病毒（WNV），分子检测，PCR，血浆）可以在特定抗体时检测来自最近WNV感染患者（即感染后3至5天）血浆中的血浆标本中的WNV RNA病毒尚未存在。然而，由于已知WNV感染的患者，检测的可能性相对较低，因为PCR检测的敏感性约为55％，血液的敏感性约为55％，血液的敏感性约为55％。

参考值
描述参考间隔和其他信息，以解释测试结果。可能会在适当的情况下包括基于年龄和性别的间隔。除非另有指定，否则间隔是梅奥衍生的。如果提供了解释性报告，则参考值字段将陈述。

IgG：负

IGM：负

参考值适用于所有年龄段

Interpretation
提供信息以帮助解释测试结果

血清中西尼罗河病毒（WNV）的IgG级抗体的存在表明过去的某个时候感染了WNV。感染后3周，几乎所有受感染的人都应开发对WNV的IgG抗体。如果怀疑怀疑急性期感染，应在感染后大约7天内与大约14至21天感染的标本进行比较，以证明2个血清样本之间的IgG抗体水平上升。

Presence of specific IgM-class antibodies in a serum specimen is consistent with acute-phase infection with WNV. By the 8th day of illness, most infected persons will have detectable serum IgM antibody to WNV; in most cases it will be detectable for at least 1 to 2 months following disease resolution and in some cases will be detectable for 12 months or longer.

The absence of IgM antibodies to WNV is consistent with lack of acute-phase infection with this virus. Specimens collected too early in the acute phase (eg, before 8 to 10 days postinfection) may be negative for IgM-specific antibodies to WNV. If WNV is suspected, a second specimen collected approximately 14 days postinfection should be tested.

In the very early stages of WNV infection, IgM may be detectable in cerebrospinal fluid (CSF) before it becomes detectable in serum.

警告
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Test results should be used in conjunction with a clinical evaluation and other available diagnostic procedures.

阴性测试结果在免疫抑制患者中的重要性尚不确定。

在过去几个月内接受输血或其他血液产品的人可能无效。

False-negative results due to competition by high levels of IgG, while theoretically possible, have not been observed.

False-positive results may occur in persons vaccinated for flaviviruses (eg, yellow fever, Japanese encephalitis, dengue)

False-positive results may occur in patients infected with other arboviruses, including flaviviruses (eg, dengue virus) and alphavirusis (eg, LaCrosse [California] Encephalitis virus, Eastern or Western equine encephalitis virus, St. Louis virus) and in persons previously infected with West Nile virus (WNV).

Because closely related arboviruses exhibit serologic cross-reactivity, it sometimes may be epidemiologically important to attempt to pinpoint the infecting virus by conducting cross-neutralization tests using an appropriate battery of closely related viruses.

WNV antibody results for cerebrospinal fluid (CSF) should be interpreted with caution. Complicating factors include low antibody levels found in CSF, passive transfer of antibody from blood, and contamination via a traumatic lumbar puncture.

临床参考
深入阅读临床性质的建议

1. Petersen LR, Marafin AA: West Nile Virus: a primer for the clinician. Ann Intern Med. 2002;137:173-179

2. Centers for Disease Control and Prevention (CDC): West Nile virus and other arboviral diseases-United States, 2012. MMWR Morb Mortal Wkly Rep. 2013;62(25):513-517

3. Brinton MA: The molecular biology of West Nile Virus: a new invader of the western hemisphere. Ann Rev Microbiol. 2002;56:371-402

4. Centers for Disease Control and Prevention (CDC): Provisional surveillance summary of the West Nile virus epidemic. United States, January-November 2002. MMWR Morb Mortal Wkly Rep. 2002;51(50):1129-1133

5.疾病控制与预防中心（CDC）：输血接受者中西尼罗河病毒感染的研究。MMWR Morb Mortal WklyRep。2002; 51（43）：973-974

Special Instructions
PDF库，包括与测试相关的相关信息和表格

Method Description
描述如何执行测试并提供特定于方法的参考

IgG:

Polystyrene microwells are coated with recombinant West Nile virus (WNV) antigen. Diluted serum specimens and controls are incubated in the wells to allow specific antibody present in the specimens to react with the antigen. Nonspecific reactants are removed by washing, and peroxidase-conjugated antihuman IgG is added and reacts with specific IgG. Excess conjugate is removed by washing. Enzyme substrate and chromogen are added, and the color is allowed to develop. After adding the stop reagent, the resultant color change is quantified by a spectrophotometric reading of optical density (OD). Specimen OD readings are compared with reference cutoff readings to determine results.(Package insert: Flavivirus [West Nile] ELISA IgG. Focus Technologies;10/16/2012)

IGM：

Polystyrene microwells are coated with the antihuman antibody specific for IgM (u-chain). Diluted serum specimens and controls are incubated in the wells, and IgM present in the specimen binds to the antihuman antibody (IgM specific) in the wells. Nonspecific reactants are removed by washing. WNV antigen is then added to the wells and incubated. If anti-WNV IgM is present in the specimen, the WNV antigen binds to the anti-WNV in the well. Unbound WNV antigen is then removed by washing the well. Mouse antiflavivirus conjugated with horseradish peroxidase (HRP) is then added to the wells and incubated. If WNV antigen has been retained in the well by the antiflavivirus in the specimen, the mouse antiflavivirus:HRP binds to WNV antigen in the wells. Excess conjugate is removed by washing. Enzyme substrate and chromogen are added, and the color is allowed to develop. After adding the Stop reagent, the resultant color change is quantified by a spectrophotometric reading of OD that is directly proportional to the amount of antigen-specific IgM present in the specimen. Specimen OD readings are compared with reference cutoff OD readings to determine results.(Package insert: Flavivirus [West Nile] IgM Capture ELISA. Focus Technologies; 06/01/2015)

PDF报告
Indicates whether the report includes an additional document with charts, images or other enriched information

不

一天表演
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

星期一，星期三，星期五

可用报告
时间间隔（在Mayo Clinic Laboratories接收样品以可用的结果）考虑yabo208到标准的设置日和周末。第一天是结果通常需要的时间。最后一天是它可能需要的时间，考虑到任何必要的重复测试。

同一天/1至4天

标本保留时间
概述了测试样品在丢弃之前将样品保存在实验室中的时间长度

14 Days

执行实验室位置
Indicates the location of the laboratory that performs the test

Rochester

费用
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • 授权用户可以登录测试价格for detailed fee information.
  • 无法访问测试价格的客户可以联系亚搏每周7天，每天24小时。
  • 潜在客户应联系其区域经理。寻求帮助，联系亚搏。

测试分类
提供有关实验室测试套件和试剂的医疗设备分类的信息。测试可能被归类为美国食品药品监督管理局（FDA）和每个制造商说明使用的清除或批准，或者用作未经全面FDA审查和批准的产品，然后被标记为Analyte特定试剂（ASR）产品。

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

IgG-86789

IGM-86788

Test Setup Resources

设置文件
测试设置信息包含测试文件定义详细信息，以支持Mayo Clinic Laboratories和您的实验室信息系统之间的订单和结果接口。yabo208

Excel|Pdf

Sample Reports
提供正常和异常样本报告作为报告外观的参考。

正常报告|Abnormal Reports

SI Sample Reports
为有限数量的测试提供了单位报告的国际系统（SI）。这些报告旨在用于国际帐户使用，只能通过定义为接收它们的Mayolink帐户提供。

SI正常报告|SI异常报告