测试ID: HCCDD
尸体或溶血标本中的丙型肝炎病毒抗体，症状，血清

ORTHO HCV Version 3.0 ELISA检测系统是一种3阶段的检测，在微孔中进行，微孔包覆重组丙型肝炎病毒(HCV)抗原(c22-3, c200和NS5)。在第一阶段，稀释的试验样品或适当的对照品在试验井中孵育一段规定的时间。如果标本中存在对三种抗原反应的抗体，则在微孔表面形成抗原-抗体复合物。如果不存在抗- hcv，则不会形成复合物。在随后的洗涤步骤中，将去除未结合的血清蛋白。在第二阶段，将与辣根过氧化物酶偶联的小鼠单克隆抗体加入微孔中。该结合物特异性地与抗原-抗体复合物中的人IgG部分结合。如果抗原-抗体复合物不存在，未结合的结合物将被随后的洗涤除去。第三阶段，在测试井中加入由邻苯二胺(OPD)和过氧化氢组成的酶检测系统。如果有结合的共轭物存在，OPD将被氧化，从而产生有颜色的最终产物。 In this reaction, peroxidase is divalently oxidized by hydrogen peroxide to form an intermediate compound, which is, in turn, reduced to its initial state by subsequent interaction with hydrogen ion donating OPD. The resulting oxidized form of OPD has an orange color. Sulfuric acid is then added to stop the reaction. The color intensity is dependent upon the amount of bound conjugate and is a function of the concentration of anti-HCV present in the specimen. The color intensity is measured with a microwell reader (photometer) designed to measure light absorbance in a microwell (Package insert: Hepatitis C Virus Encoded Antigen [Recombinant c22-3, c200, and NS5], ORTHO HCV Version 3.0 ELISA Test System. Ortho Clinical Diagnostics; 01/2017)

星期一,星期四